Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1215651 | Journal of Chromatography B | 2007 | 8 Pages |
Abstract
We describe a new fully validated enantioselective LC–MS/MS method for stereospecific quantification of both the racemic forms of Warfarin (WF), Phenprocoumon and Acenocoumarol in human plasma. Measurement specificity was assessed by using different blank donor plasma samples, where no interfering reagent peak appeared at the retention time (RT) of the targeted analytes. Response was linear for all analytes. Typical linear regression coefficients have >0.99. The recoveries ranged from 98% to 118%. Determinations in 10 normal healthy individuals revealed a high reproducibility of RTs. These findings confer to the method suitability for large population studies.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Gennaro Vecchione, Bruno Casetta, Michela Tomaiuolo, Elvira Grandone, Maurizio Margaglione,