| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1215823 | Journal of Chromatography B | 2007 | 6 Pages |
Abstract
A competitive immunoassay for detecting clenbuterol in urine was established by capillary electrophoresis (CE) with laser-induced fluorescence (LIF). The clenbuterol was conjugated with bovine serum albumin (BSA), and then the derivative was labeled with fluorescein isothiocyanate (FITC) and competes for antibody with free clenbuterol in the sample. Under the optimal conditions, Free and bound FITC labeled clenbuterol was separated within 8 min with the relative standard deviation (R.S.D.) 0.72% for migration time and 2.8% for peak area. The detection limit reached 0.7 ng/ml.
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Authors
Jieyu Zhou, Xueshu Xu, Yuxiao Wang,