| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1215981 | Journal of Chromatography B | 2009 | 6 Pages |
Abstract
In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. In addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Carl Grey, Per Edebrink, Maria Krook, Sven P. Jacobsson,