| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1216327 | Journal of Chromatography B | 2007 | 7 Pages |
Abstract
We examined the influence of oxidative stress on the relative amounts of various albumin-bound thiols in human plasma. To determine the ratio of thiols existing as mixed disulfides following oxidation, we developed a method combining fast purification of albumin using affinity columns and high-performance liquid chromatography (HPLC) with fluorescence detection for low molecular weight thiols which were labeled after reduction.When the effect of exposure of plasma to radical oxygen species on binding of thiols to albumin was determined by the present method, significant increases in the ratio of cysteine bound to albumin (Alb-Cys) to total cysteine were clearly demonstrated.
Related Topics
Physical Sciences and Engineering
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Authors
Yuki Ogasawara, Yoshio Mukai, Tadayasu Togawa, Toshihiro Suzuki, Shinzo Tanabe, Kazuyuki Ishii,