| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1216330 | Journal of Chromatography B | 2007 | 6 Pages |
Abstract
A racemate from natural product, tetrahydropalmatine (THP), was characterized on its enantioselective binding to DNA by the chromatographic methods including microdialysis/HPLC, centrifugal ultrifiltration/HPLC and immobilized DNA affinity chromatography. It was found that its (+)-enantiomer was preferential to binding on B-form duplex DNA including calf thymus DNA, AT and GC sequence oligo DNA, as well as triplex oligo DNA. The binding constants of the THP enantiomers to ct-DNA were determined with the methods of microdialysis/HPLC and frontal affinity chromatography. In addition, the DNA structural preference of either enantiomer was evaluated with the chromatographic methods.
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Authors
Xingye Su, Feng Qin, Liang Kong, Junjie Ou, Chuanhui Xie, Hanfa Zou,