| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1216397 | Journal of Chromatography B | 2006 | 8 Pages |
A method for the quantitation of DB-67 ((20S)-10-hydroxy-7-tert-butyldimethylsilylcamptothecin) lactone and carboxylate in mouse plasma has been developed, validated, and applied in pharmacokinetic studies. The analytes were separated by reversed-phase chromatography with fluorescence detection. Validation demonstrated the selectivity and specificity for the carboxylate and lactone, with linearity between 1–300 ng/mL and 2.5–300 ng/mL for the carboxylate and lactone, respectively (accuracy 90–110% of theory and coefficient of variation ≤5.7%). Carboxylate to lactone conversion was <4% using this method. The assay was found to be suitable for the analysis of DB-67 lactone and carboxylate in pharmacokinetic studies following intravenous administration of DB-67 or its δ-aminobutyric acid ester derivative.
