Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1216441 | Journal of Chromatography B | 2009 | 7 Pages |
Abstract
A rapid high performance liquid chromatographic method was developed including an internal standard for the measurement of mature and senescent crosslinks concentration in non-demineralized bone hydrolysates. To avoid the demineralization which is a tedious step, we developed a method based on the use of a solid-phase extraction procedure to clean-up the samples. It resulted in sensitive and accurate measurements: the detection limits as low as 0.2 pmol for the pyridimium crosslinks and 0.02 pmol for the pentosidine. The inter- and intra-assay coefficients of variation were as low as 5% and 2%, respectively, for all crosslinks.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
S. Viguet-Carrin, E. Gineyts, C. Bertholon, P.D. Delmas,