| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1216556 | Journal of Chromatography B | 2006 | 5 Pages |
Abstract
Blackcurrant (Ribes nigrum) seed oil is rich in α- and γ-linolenic acids, the latter in particular being of potential use in medicine. The enzymatic hydrolysis of the oil was carried out in supercritical carbon dioxide using lipase Lipozyme as catalyst and changes in the composition of acylglycerols were recorded. Mono-, di-, and triacylglycerols and free fatty acids were separated by non-aqueous high-performance liquid chromatography in reversed phase mode and detected by UV diode array and 1H NMR detectors. Lipozyme was found to exert low specificity to individual fatty acids in the hydrolysed oil.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Milena Bártlová, Prokop Bernášek, Jan Sýkora, Helena Sovová,