Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1216626 | Journal of Chromatography B | 2008 | 8 Pages |
Abstract
NMR-based metabonomics is a valuable and straightforward approach to measuring hundreds of metabolites in complex biofluids. However, metabolite identification is sometimes limited by overlapped signals in NMR spectra. We describe a new methodology using an automated hyphenation of solid phase extraction (SPE) with RP-HPLC combined to NMR spectroscopy, which allowed identification of 72 metabolites of various molecular classes in human urine. This methodology was also successfully applied to the fractionation of a cat urine sample to aid identification of aromatic compounds and felinine. The SPE-RP-HPLC method appears to be a reliable tool to support biomarker discovery in metabonomic studies.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Serge Rezzi, Francia Arce Vera, François-Pierre J. Martin, Serena Wang, Dennis Lawler, Sunil Kochhar,