| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1216953 | Journal of Chromatography B | 2008 | 7 Pages |
Abstract
Ion-exchange chromatography has been applied to purification of unsaturated oligoglucuronans. After an isocratic elution on a strong anion-exchange column, the collected fractions were desalted by low pressure size exclusion chromatography. However, this efficient separation was limited by the time required to desalt. So, we developed a reversed-phase chromatography method using back ionization of oligomers. Two C18 columns were tested with trifluoroacetic acid (TFA 0.7%) as eluent. Different selectivities and column stabilities were observed in this acidic condition. The scale up for semi-preparative applications enabled us to recover pure unsaturated oligoglucuronans without desalting step.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Marie-Laure Tavernier, Emmanuel Petit, David Lesur, Anne Wadouachi, Bernard Courtois, Josiane Courtois, Philippe Michaud,