Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1216955 | Journal of Chromatography B | 2008 | 10 Pages |
Benzene and toluene are important industrial chemicals and ubiquitous environmental pollutants. The urinary mercapturic acids of benzene and toluene, S-phenylmercapturic acid (S-PMA) and S-benzylmercapturic acids (S-BMA) are specific biomarkers for the determination of low-level exposures. We have developed and validated a fast, specific and very sensitive method for the simultaneous determination of S-PMA and S-BMA in human urine using an automated multidimensional LC–MS–MS-method that requires no additional sample preparation. Analytes are stripped from urinary matrix by online extraction on a restricted access material, transferred to the analytical column and subsequently determined by tandem mass spectrometry using isotopically labelled S-PMA as internal standard. The lower limit of quantification (LLOQ) for both analytes was 0.05 μg/L urine and sufficient to quantify the background exposure of the general population. Precision within series and between series for S-PMA and S-BMA ranged from 1.0% to 12.2%, accuracy was 108% and 100%, respectively. We applied the method on spot urine samples of 30 subjects of the general population with no known exposure to benzene or toluene. Median levels (range) for S-PMA and S-BMA in non-smokers (n = 15) were 0.14 μg/L (<0.05–0.26 μg/L) and 8.2 (1.6–77.4 μg/L), respectively. In smokers (n = 15), median levels for S-PMA and S-BMA were 1.22 μg/L (0.17–5.75 μg/L) and 11.5 μg/L (0.9–51.2 μg/L), respectively. Due to its automation, our method is well suited for application in large environmental studies.