High-performance liquid chromatography spectrometric analysis of tripterin in rat plasma

A quick, precise and reliable HPLC method has been developed to determine tripterin in rat plasma. After liquid–liquid extraction, the analytes was analyzed on a Discovery ODS C18 column (5 μm, 4.6 mm × 250 mm) with an isocratic elution consisting of methanol–water–phosphoric acid (87:13:0.2, v/v/v). Ultraviolet detection was at 425 nm. Using trioxymethylanthraquinone as an internal standard, the assay was linear over the concentration range of 0.025–1.60 μg/mL (r2 = 0.9988). The extraction recovery of tripterin in rat plasma was more than 62%. The intra- and inter-day precision was less than 13% (CV). This validated method was successfully applied to the pharmacokinetics of tripterin in rats.