| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1217017 | Journal of Chromatography B | 2012 | 8 Pages |
Triacylglycerides (TAGs) are a large class of complex neutral lipids that naturally occur in both plants and animals. In the present work, an on-line comprehensive silver-ion liquid chromatography (silver-ion LC) × reversed-phase liquid chromatography (RPLC) system was constructed to analyze these compounds. A micro bore silver-ion modified column was employed in the first dimension with the commonly used hexane-based mobile phase. After a series of C18 columns were assessed, a wide bore column packed with 1.5 μm particles was selected as the second dimension column to reduce the negative effect caused by the large volume and strong solvent injection in the second dimension. The system coupled with mass spectrometry was applied to the analysis of an edible peanut oil and a mouse liver extract. Twenty-eight TAGs from the peanut oil and forty-four from the mouse liver were identified based on the TAGs’ retention behaviors on the comprehensive two-dimensional LC system and their APCI MS fragments.
► An on-line comprehensive Ag+ × RP liquid chromatography system was constructed. ► The second dimensional column was optimized to get better LC × LC separations. ► 28 TAGs from peanut oil and 44 TAGs from mouse liver were identified. ► Identifications were based on TAGs’ retention behaviors and their MS fragments.
