Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1217187 | Journal of Chromatography B | 2007 | 4 Pages |
Abstract
A gradient reversed-phase HPLC assay has been developed to determine sodium ferulate (SF) in beagle dog plasma with tinidazole as an internal standard. Chromatographic separation was made on a C18 column using 0.5% acetic acid and acetonitrile (80:20, v/v) as mobile phase. UV detection was performed at 320 nm. The calibration curve for SF was linear in the range of 0.05–10 μg/ml, and the achieved limit of quantification (LOQ) was 51.4 ng/ml. The results of linearity, within- and between-day precision, and accuracy demonstrate that this method is reliable, sensitive and sufficient for in vivo beagle dog pharmacokinetic (PK) studies of SF.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Feng-Qian Li, Shu Xu, Hua Su, Jia-Xin Deng, Ji-Yong Liu, Jin-Hong Hu,