Article ID Journal Published Year Pages File Type
1217269 Journal of Chromatography B 2006 5 Pages PDF
Abstract

A simple and sensitive method was developed for the quantification of amikacin in human plasma and urine samples. The method involves centrifugation of body fluid plasma after dilution with an ethanol/sodium carbonate mixture, and then an aliquot of the supernatant is directly injected into the chromatograph. After separation on a reversed-phase C18 column (runtime 20 min), aminoglycoside is detected on the basis of its complex formation reaction with Cu(II), the catalyst of the luminol/hydrogen peroxide chemiluminescence system. Using a volume of 500 μl biological sample, linearity is established over the concentration range 0.15–2.0 μg/ml and the limit of detection (LOD) is ca. 50 μg/l in plasma or urine. The intra-day and inter-day precision (measured by relative standard deviation, R.S.D.%) are always less than 9%, and relative recoveries are found to be over 92%.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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