| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1217556 | Journal of Chromatography B | 2009 | 5 Pages |
Abstract
A LC/MS/MS method was developed and validated for determination of nobiliside A in rat plasma. Analyses were separated by an Agella Venusil ASB-C18 column and isocratic elution with methanol:water (80:20, v/v) as a mobile phase. A tandem mass spectrometer was used as a detector for quantitative analysis. Calibration curves (R2 > 0.999) in spiked plasma were linear over the concentration range of 50–5000 ng/mL. The overall accuracy of this method was 93–104% for nobiliside A. Within-day and between-day variability was both less than 9% in plasma. The data indicate that the LC/MS/MS method is an effective method for the pharmacokinetics study of nobiliside A in rat plasma.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Dan Guo, Yang Xiong, Yue Zhang, Zhongbin Wu, Lili Cui, Jianming Chen,