Miniaturized hollow fiber assisted liquid-phase microextraction and gas chromatography-mass spectrometry for the measurement of progesterone in human serum

Miniaturized hollow fiber assisted liquid-phase microextraction (HF-LPME) and gas chromatography-mass spectrometry (GC-MS) for the determination of progesterone in human serum sample is described. The detection limit and the quantification limit of progesterone in human serum sample are 0.5 and 2 ng mL−1 (ppb), respectively. The calibration curve for progesterone is linear with a correlation coefficient of >0.999 in the range of 2-500 ng mL−1. The average recoveries of progesterone in human serum samples spiked with 5, 50 and 200 ng mL−1 progesterone are 97.4% (R.S.D.: 9.3%), 100.3% (R.S.D.: 4.7%) and 99.8% (R.S.D.: 3.4%), respectively. This simple, accurate, sensitive and selective analytical method can be applicable for the determination of progesterone in human serum samples.