| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1217618 | Journal of Chromatography B | 2008 | 5 Pages |
Abstract
A reliable and validated LC–MS method was established for determination of r-RGD-Hirudin in human serum. Ultrafiltration was used instead of liquid–liquid extraction or solid phase extraction for water solubility drug r-RGD-Hirudin extraction. Freeze drying was used for concentration. The experiment conditions, including pre-processing procedure and LC–MS, have been investigated and optimized. Comparing with reported assays, the current method showed significant improvement in specificity, linearity, precision and sensitivity. This method has been successfully applied in clinical research of r-RGD-Hirudin.
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Authors
Shengmin Su, Yunqiu Yu, Wei Mo, Yanling Zhang, Houyan Song, Qinfen Chen, Yi Xie,