Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1217708 | Journal of Chromatography B | 2007 | 7 Pages |
A parallel chromatographic procedure for the purification of milligram amounts of plasmid DNA was developed. Initial studies showed that ion-exchange membrane capsules displayed high capacity for plasmid DNA. Interestingly, a weak anion exchanger (DEAE) proved to be superior to the strong quarternary ammonium group with respect to elution and regeneration properties and the 75 cm2 Sartobind D membrane capsule (MA75D, Sartorius) was selected for further studies. A method for reducing endotoxin levels by using CTAB as a precipitant was optimised. By introducing this step into the protocol, endotoxin levels could be reduced approximately 100-fold to ≤5 EU/mg plasmid. The parallel procedure was set up on a multi-channel peristaltic pump and evaluated with four different vectors (2.7–11.5 kbp). Starting with 5–10 g of E. coli cell paste (wet weight) generally saturated the membrane adsorber, resulting in plasmid DNA yields close to 10 mg.