| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1217735 | Journal of Chromatography B | 2007 | 6 Pages |
Abstract
A validated, quantitative LC–APCI-MS/MS method for methadone, EDDP and EMDP in 200-μL plasma is presented. Specimen preparation was limited to protein precipitation and centrifugation. Chromatographic separation was achieved on a Synergi Hydro-RP 80A (50 mm × 2.0 mm, 4 μm) column with gradient elution. The assay was linear from 1 to 500 ng/mL, with intra- and inter-assay accuracy ≥87.5% and intra- and inter-assay precision <13.4% R.S.D. and recovery ≥87.5% for all analytes at 40 ng/mL. This analytical method is suitable for the accurate and precise determination of methadone and metabolites in human plasma specimens.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Diaa M. Shakleya, Lauren M. Jansson, Marilyn A. Huestis,