Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1217807 | Journal of Chromatography B | 2007 | 9 Pages |
Abstract
The lead optimization phase of drug discovery requires high-throughput analyses for quantification in biological matrices and in plasma in particular. Over the last decade, some technical innovations allowed the pharmaceutical industry to improve the quality of the results. However, there is room for improvement. In this context, a new calibration strategy is proposed in this paper. Experiments were performed on dog plasma samples and it was showed that a within-animal calibration strategy can reduce the bias up to 20% and improve the precision up to 20%. However, a partial within-animal calibration is preferred to the full approach in order to avoid to many sample preparations.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Walthère Dewé, Michaël De Smet, Nathalie Evrard, Benoit Culot, Marc Lastelle, Gaëlle Saucez, Dominique Ingels, Xavier Taillieu,