HPLC quantification of perphenazine in sheep plasma: Application to a pharmacokinetic study

A high performance liquid chromatographic (HPLC) method for the determination of perphenazine (PPZ) in sheep plasma was developed and validated. The separation was achieved using a 5 μm C18 column (125 mm × 4 mm) with a mobile phase composed of acetonitrile and an aqueous solution of H3PO4 and TBA (inverse gradient). The flow rate was 1.5 mL/min and the UV detection was performed at 258 nm. The method was validated with respect to linearity, intra and inter-day precision and accuracy, limit of quantification, limit of detection and storage stability. This method was used to perform a pilot pharmacokinetic study of PPZ after subcutaneous administration to one ewe.