| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1217827 | Journal of Chromatography B | 2007 | 4 Pages |
Abstract
In this paper, a rapid method based on high-performance liquid chromatography/electrospray–mass spectrometry (HPLC/ESI-MS) method for the quantitative determination of andrographolide (AND) in human plasma has been developed and validated. A liquid–liquid extraction (LLE) procedure was selected to isolate AND from biological matrixes. Isosorbide-5-mononitrate (IS-5-MN) was selected as the internal standard (IS). The correlation coefficient of the calibration curve was 0.998, in the range of 9.9–320.0 ng/mL. The validated method may be used to assess the bioavailability and pharmacokinetics of the drug.
Related Topics
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Authors
Yanli Gu, Jinyu Ma, Yali Liu, Bo Chen, Shouzhuo Yao,