Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1217860 | Journal of Chromatography B | 2007 | 11 Pages |
Abstract
In order to separate hydrophobic membrane proteins, we have developed a novel two-dimensional electrophoresis system. For the iso-electric focusing, agarose was used as a supporting matrix and n-dodecyl-β-d-maltopyranoside was used as a surfactant. In combination with a previously developed Tris/MES electrophoresis system in the second dimension, distinct spots were reproducibly detected from hydrophobic membrane proteins whose grand average hydropathicity (GRAVY) exceed 0.3. In contrast to the immobilized pH gradient system, c-type heme was also visualized in this system.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yasuhiro Kashino, Takeshi Harayama, Himadri B. Pakrasi, Kazuhiko Satoh,