Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1217899 | Journal of Chromatography B | 2007 | 5 Pages |
Abstract
Glucose-6-phosphate dehydrogenase (G6PD) present in Saccahromyces cerevisiae is an enzyme of the pentose pathway. An effective enrichment of this intracellular enzyme can be achieved with the reversed micellar methodology. In this work, this methodology was employed with soybean lecithin, a biocompatible surfactant. A factorial design was used to evaluate the influence of pH (A) and extraction runs (B) on the G6PD purification factor. After statistical analysis and process optimization, a mathematical model representing G6PD enrichment was obtained: Y = 4.89 − 0.83A + 0.092B + 0.27AB − 1.37B2 with an enzyme purification factor of about 5.2.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Francislene Andréia Hasmann, Daniela de Borba Gurpilhares, Inês Conceição Roberto, Adalberto Pessoa Jr.,