Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1217916 | Journal of Chromatography B | 2007 | 7 Pages |
Abstract
Mass spectrometry is a powerful tool for studying the intracellular pharmacokinetics of antiretroviral drugs. However, the biohazard of HIV-1 calls for a safety protocol for such analyses. To this end, we extracted HIV-1 producing cells with methanol or ethanol at 4 °C. After extraction, no viral infectivity was detected, as shown by a reduction in infectious titers of more than 6 log. In addition, this protocol is compatible with the quantitative analysis of antiretroviral drugs in cell extracts using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. Thus, using this protocol, infectious HIV-1 is inactivated and antiretroviral drugs are extracted from cells in a single step.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jeroen J.A. van Kampen, Esther J. Verschuren, Peter C. Burgers, Theo M. Luider, Ronald de Groot, Albert D.M.E. Osterhaus, Rob A. Gruters,