| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1217965 | Journal of Chromatography B | 2006 | 5 Pages |
Abstract
A simple HPLC–UV method was developed for the determination of scutellarin in plasma and different tissues of mice (heart, liver, spleen, lungs and kidneys). The separation was achieved by HPLC on a Hypersil C18 column with a mobile phase composed of methanol–water–glacial acetic acid (40:60:1). UV detection was used at 335 nm. The calibration curves were linear in all matrices (r2 > 0.997) in the concentration range of 0.1–10 μg/ml for plasma and 0.1–20 μg/g for tissue homogenates, respectively. The method described is suitable for studies on the distribution of scutellarin in different tissues of mice.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Fei Xiong, Hao Wang, Ji Cheng, Jiabi Zhu,