Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1217997 | Journal of Chromatography B | 2006 | 4 Pages |
An HPLC procedure developed for the rapid and simultaneous determination of purine derivatives (PD) in ruminants’ urine was investigated, since the adoption of a single method for the simultaneous detection of PD and creatinine was not carried out due to elution of polar co-extractives and also due to overlapping of the peaks of allantoin and creatinine. The experimental conditions chosen in the present study avoid the presence of chemically competitive compounds and afford a good separation of the peaks of allantoin and creatinine. The recoveries of the standard compounds added to urine samples were 94–104%. This method can be proposed as a possible reference method for the estimation of allantoin, uric acid and creatinine in cattle urine.