Article ID Journal Published Year Pages File Type
1217997 Journal of Chromatography B 2006 4 Pages PDF
Abstract

An HPLC procedure developed for the rapid and simultaneous determination of purine derivatives (PD) in ruminants’ urine was investigated, since the adoption of a single method for the simultaneous detection of PD and creatinine was not carried out due to elution of polar co-extractives and also due to overlapping of the peaks of allantoin and creatinine. The experimental conditions chosen in the present study avoid the presence of chemically competitive compounds and afford a good separation of the peaks of allantoin and creatinine. The recoveries of the standard compounds added to urine samples were 94–104%. This method can be proposed as a possible reference method for the estimation of allantoin, uric acid and creatinine in cattle urine.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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