A new derivatization reagent for determination of biogenic amines in wines

•New derivatization procedures of biogenic amines were developed.•1-Fluoro-2-nitro-4-(trifluoromethyl)benzene was used for derivatization.•Time for sample preparation and derivatization was minimized.•The tested procedures were applied for the determination of biogenic amines in wines.•The developed procedure can be employed in food industry.

Fermented beverages are the main source of biogenic amines in our diet. Their levels are very difficult to minimize and important for the control of wine quality. Some of them are harmful to human health and methods of their analysis are still developed. In this paper, a new reagent, 1-fluoro-2-nitro-4-(trifluoromethyl)benzene, for precolumn derivatization of selected biogenic amines, and their chromatographic determination is described. The derivatives of histamine, tryptamine, tyramine and phenylethylamine were synthesized in high purity and characterized by 1H, 13C and 19F NMR. A convenient procedure based on the 1-fluoro-2-nitro-4-(trifluoromethyl)benzene has been developed for the determination of these amines in wine samples. The coefficient of variation, <2.5% for all samples, the recovery ≈100%, demonstrate repeatability and accuracy of procedure. It is convenient, shows lower solvent consumption, and takes less time in comparison to previously described methods.