Ancient apple varieties from Croatia as a source of bioactive polyphenolic compounds

•Polyphenols were analysed in the flesh and peel of ancient apple varieties.•Apples varied in polyphenol content.•Wild apple, Zimnjara, Lještarka, Adamova zvijezda had high polyphenol content.•Flesh can be classified by the portion of phenolic acids or flavanols.•A pattern for the classification of the peel was not observed.

Several ancient apple varieties and a wild apple variety grown in Croatia were analysed for the polyphenol content and compared to two varieties grown in USA. In the flesh, flavanols, dihydrochalcones and phenolic acids (24 to 137, 23 to 109, 3 to 238 mg kg−1 of fresh weight (FW), respectively) were found. Peel contained flavanols, dihydrochalcones, phenolic acids, flavonols and anthocyanins (65 to 690, 21 to 141, 0 to 107, 205 to 1223, 0 to 213 mg kg−1 FW, respectively). The wild apple was characterized by much higher flavanol and phenolic acid content in the flesh (301 and 734 mg kg−1 FW, respectively) while the peel was similar to other apples. The polyphenol profile was similar to apples from USA. The varieties Zimnjara, Lještarka and Adamova zvijezda could be highlighted as sources of polyphenols. Varieties are categorized by the content of dihydrochalcones and flavanols in the flesh (whether that content is high or low), and by the relative portion of phenolic acids and flavanols in the flesh (high phenolic acid proportion, lower flavanol proportion and vice versa). There was not observed to be as strong a pattern for categorizing differences in the peel.