Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1220474 | Journal of Pharmaceutical and Biomedical Analysis | 2015 | 6 Pages |
•We determined d-alanine content in kuruma prawn during hyper-osmotic stress.•d-Alanine in the hepatopancreas increased along with hyper-osmotic stress.•Alanine racemase activity in the hepatopancreas elevated during hyper-osmotic stress.•d-Alanine content is controlled by the gene expression level of alanine racemase.
Changes in d- and l-alanine contents were determined in the muscle and hepatopancreas of kuruma prawn Marsupenaeus japonicus, during acclimation from seawater containing 100% salinity to artificial seawater containing 150% salinity. In the hepatopancreas, contents of both amino acids increased by approximately threefold. The activity of alanine racemase, which catalyzes the interconversion of d- and l-alanine, also increased in the high-salinity seawater. In addition, the expression of the gene encoding alanine racemase increased in the hepatopancreas with an increase in the alanine racemase activity. These data indicate that the biosynthesis of d- and l-alanine is controlled by the gene expression level of alanine racemase, and d-alanine in the hepatopancreas functions as a major osmolyte for isosmotic regulation. In contrast, the content of d-alanine and alanine racemase activity did not change in the muscle during hyper-osmotic acclimation. Therefore, we suggest that d-alanine, which exists in the several tissues of M. japonicus, is considered to be utilized in some different physiological phenomena in different tissues.
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