Simultaneous determination of insulin and its analogues in pharmaceutical formulations by micellar electrokinetic chromatography

•A MEKC method is proposed to identify human insulin and its five analogues.•The major degradation product of each insulin can also be separated.•The CE method is validated for the quantification of human insulin.•The quality control of several pharmaceutical formulations has been performed.

A simple and efficient MEKC method was developed to simultaneously determine human insulin, its five analogues, the main degradation products and the excipients usually present in injection formulations. A very fast method with a total analysis time of 3 min was then successfully validated for the analysis of human insulin and the quality control of commercial formulations was carried out.