Application of two-phase hollow fiber liquid phase microextraction coupled with high-performance liquid chromatography for the study of the echinacoside pharmacokinetics in Parkinson's disease rat plasma

•Based on HF-LPME technique has been developed.•The HF-LPME was investigated detailedly.•The first report HP-LPME for the analysis of ECH in Parkinson's disease rat plasma.

A simple and solvent-minimized sample preparation technique based on two-phase hollow fiber liquid phase microextraction (HF-LPME) was developed and used to quantify the echinacoside in Parkinson's disease rat plasma following oral administration. Analysis was accomplished by reversed-phase high performance liquid chromatography (HPLC) with ultraviolet detection. The influence factors relevant to the HF-LPME processes were optimized. Under the optimized conditions, the preconcentration factor for echinacoside was 337. Calibration curves with reasonable linearity (r2 ≥ 0.9998) were obtained in the range of 5–750 ng mL−1. Intra-day and inter-day precision (RSD) were ≤5.43% and the limit of detection (LOD) for the analyte was 2.0 ng mL−1 (S/N = 3). The validated method has been successfully applied for pharmacokinetic studies of echinacoside in Parkinson's disease (PD) rat plasma after oral administration.

Graphical abstractTypical chromatograms of determination of echinacoside (50 ng mL−1) and IS in plasma samples; (A) plasma sample 1 h after oral administration with liquid–liquid extraction; (B) plasma sample 1 h after oral administration with two-phase hollow fiber liquid phase microextraction.Figure optionsDownload full-size imageDownload as PowerPoint slide