| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1221364 | Journal of Pharmaceutical and Biomedical Analysis | 2014 | 6 Pages |
•Development of a UHPLC–MS/MS method to determine HPABA in rat plasma.•The total analysis time was 2.0 min.•The method was applied to pharmacokinetic study of HPABA in rats.•HPABA showed linear pharmacokinetic properties and high absolute bioavailability.
A rapid, sensitive and high throughput UHPLC–MS/MS method was established and validated to assay the concentration of 2-(2-hydroxypropanamido) benzoic acid (HPABA), a promising anti-inflammatory drug, in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate and separated on a Shim-pack XR-ODS C18 column (75 mm × 3.0 mm, 2.2 μm) at an isocratic flow rate of 0.4 mL/min using acetonitrile–0.1% formic acid in water (50:50, v/v) as mobile phase, and total run time was 2 min. MS/MS detection was accomplished in multiple reaction monitoring (MRM) mode with positive electrospray ionization. The calibration curve was linear over the concentration range of 0.01–50 μg/mL with lower limit of quantification of 0.01 μg/mL. The intra- and inter-day precisions were below 8.5% in terms of relative standard deviation (RSD), and the accuracy was within ±4.0% in terms of relative error (RE). Extraction recovery, matrix effect and stability were satisfactory in rat plasma. The developed method was successfully applied to a pharmacokinetic study of HPABA following intragastric administration of 25, 50, 100 mg/kg and an intravenous injection at a dose of 12.5 mg/kg to Sprague-Dawley rats. Results indicated that HPABA had linear pharmacokinetic properties within the tested intragastric dosage range and the absolute bioavailability was above 59.1%.
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