| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1221491 | Journal of Pharmaceutical and Biomedical Analysis | 2014 | 6 Pages |
•LC–MS method was developed for simultaneous determination of plantamajoside and acteoside in rat plasma.•The fully validated method was applied to pharmacokinetic study.•Pharmacokinetics of two analytes in rats administered with Plantago asiatica was firstly reported.
Plantago asiatica is a medicinal and dietary plant rich in polyphenolic compounds such as phenylpropanoid glycosides plantamajoside and acteoside. The aims of the present study were to develop a new and sensitive method for simultaneous determination of plantamajoside and acteoside and investigate their pharmacokinetic properties in rats. A sensitive and rapid liquid chromatography–tandem mass spectrometry (LC–MS/MS) method with multiple-reaction monitoring (MRM) mode was employed for quantification of two analytes in rat plasma. The calibration curve was linear over the range of 0.2–200 ng/ml with correlation coefficient greater than 0.9983 for both analytes. The accuracy of plantamajoside and acteoside were between −4.2% and 8.1%, −3.8% and 8.9% relative error, respectively. Precision for the two analytes ranged from 2.7 to 10.2% relative standard deviation. The pharmacokinetic results showed plantamajoside and acteoside were quickly absorbed in rat with the time to maximum plasma concentration 16.7 ± 2.8 and 13.3 ± 2.8 min, respectively. The elimination constants were 0.28 ± 0.01 1 h−1 for plantamajoside and 0.47 ± 0.03 1 h−1 for acteoside. The developed method and the pharmacokinetic data provide a basis for further studies on bioactivity of P. asiatica.
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