| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1221885 | Journal of Pharmaceutical and Biomedical Analysis | 2013 | 10 Pages |
•The azithromycin aglycone and its derivatives were analyzed by ESI-MSn.•The fragmentation reactions of macrocycles were studied by H/D exchange and HRMS.•The fragmentation pathways of [M+H]+ and [M+Na]+ ions were proposed.
A comprehensive study of fragmentation pathways of 15-membered azalide macrocycles using electrospray ionisation with multistage mass spectrometry (ESI-MSn) is presented in this work. Hydrogen/deuterium (H/D) exchange experiments and high-resolution mass spectrometry were used to investigate the proposed fragmentation pathways. In addition, the fragmentation patterns of sodium adduct ions [M+Na]+ of the macrocycles were also investigated as the presence of an alkali metal interacting with the aglycone ring influences the product-ion spectra.
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