Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLC-PAD

The paper describes the development of a simultaneous determination method for polar and non-polar ginsenosides in red ginseng with a reversed-phase high-performance liquid chromatography-pulsed amperometric detection method. This method could be applied directly without any pretreatment steps and enabled the performance of highly sensitive analysis within 1 h. The detection (S/N = 3) and quantification (S/N = 10) limits for the ginsenosides ranged 0.02–0.10 ng and 0.1–0.3 ng, respectively. The linear regression coefficients ranged 0.9975–0.9998. Intra- and inter-day precisions were <9.91%. The mean recoveries ranged 98.08–103.06%. The total amount of ginsenosides in the hairy root of red ginseng was higher than that in the main root.