| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1222175 | Journal of Pharmaceutical and Biomedical Analysis | 2012 | 6 Pages |
A high performance liquid chromatography coupled with diode array detector (HPLC-DAD) method was developed for simultaneous quantification of eleven major bioactive components including six coumarins, three flavonoids and two limonoids in Fructus Citri Sarcodactylis. The analysis was performed on a Cosmosil 5 C18-MS-II column (4.6 mm × 250 mm, 5 μm) with water–acetonitrile gradient elution. The method was validated in terms of linearity, sensitivity, precision, stability and accuracy. It was found that the calibration curves for all analytes showed good linearity (R2 > 0.9993) within the test ranges. The overall limit of detection (LOD) and limit of quantification (LOQ) were less than 3.0 and 10.2 ng. The relative standard deviations (RSDs) for intra- and inter-day repeatability were not more than 4.99% and 4.92%, respectively. The sample was stable for at least 48 h. The spike recoveries of eleven components were 95.1–104.9%. The established method was successfully applied to determine eleven components in three samples from different locations. The results showed that the newly developed HPLC-DAD method was linear, sensitive, precise and accurate, and could be used for quality control of Fructus Citri Sarcodactylis.
► Development of an HPLC method for simultaneous quantification of eleven major bioactive components including six coumarins, three flavonoids and two limonoids in Fructus Citri Sarcodactylis. ► Different wavelengths were chosen to analyze different type of compounds. ► Optimization of the HPLC conditions. ► Validation of the method (linearity, limit of detection and quantification, precision, recovery and stability).
