| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1222309 | Journal of Pharmaceutical and Biomedical Analysis | 2012 | 7 Pages |
Crude phenolic extracts (PE) have been obtained from naturally bearing Spanish extra-virgin olive oil (EVOO) showing different polyphenol families such as secoiridoids, phenolic alcohols, lignans, and flavones. EVOO-derived complex phenols (especially from the Arbequina variety olive) have been shown to suppress cell growth of SW480 and HT29 human colon adenocarcinoma cell lines. Inhibition of proliferation by EVOO-PE Arbequina variety extract was accompanied by apoptosis in both colon-cancer-cell lines and a limited G2M cell-cycle arrest in the case of SW480 cells. The metabolized compounds from EVOO-PE in culture medium and cytoplasm of both cell lines were analyzed using nano-liquid chromatography (nanoLC) coupled with electrospray ionization-time-of-flight-mass spectrometry (ESI-TOF-MS). The results showed many phenolic compounds and their metabolites both in the culture medium as well as in the cytoplasm. The main compounds identified from EVOO-PE were hydroxylated luteolin and decarboxymethyl oleuropein aglycone.
► NanoLC-ESI-TOF-MS is applied to the metabolomic analysis of colon-cancer-cell lines. ► The main compounds identified were hydroxylated luteolin and DOA. ► Extra-virgin olive oil suppresses cell growth of SW480 and HT29 cell lines. ► Inhibition of proliferation is accompanied by apoptosis in both cell lines. ► The cytostatic effect of olive oil on SW480 cells is indicated by cell arrest in G2M.
