| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1222595 | Journal of Pharmaceutical and Biomedical Analysis | 2011 | 7 Pages |
Abstract
A rapid procedure for the determination of lysine based on hydrophilic interaction chromatography (HILIC) separation of arginine and lysine with fluorescence detection has been developed. The separation conditions and parameters of lysine postcolumn derivatization with o-phtaldialdehyde (OPA)/2-mercaptoethanol were studied. The various HILIC columns were employed using isocratic elution. Fluorescence detection was performed at excitation and emission wavelength of 345 nm and 450 nm, respectively. An advantage of the reported method is a simple sample pre-treatment and a quick and very sensitive HPLC method. The developed method was successfully applied for analysis of commercial samples of Ibalgin Fast tablets (Zentiva, Czech Republic).
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Michal Douša, Jiří Břicháč, Petr Gibala, Petr Lehnert,