Liquid chromatographic analysis of guanidino compounds using furoin as a new fluorogenic reagent

Furoin, a benzoin analogue, was examined as novel fluorogenic reagent for the selective and sensitive LC determination of various guanidines after pre-column derivatization. The derivatization reaction was carried out at 100 °C for 5 min to give adducts that were separated on a Phenomenex Synergi MAX-RP column and detected at λem = 410 nm with λex = 325 nm. The reagent showed to be useful both for determining together arginine (Arg) and creatine (CT) in dietary supplements under elution isocratic conditions and for the simultaneous analysis of a variety of guanidines in biological samples (human plasma and urine) under elution gradient conditions. The detection limits ranged from 7 to 25 fmol. Recovery studies showed good results for all determined guanidino compounds (85.6–106.2%; R.S.D. = 1.1–6.2%).