Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1222948 | Journal of Pharmaceutical and Biomedical Analysis | 2011 | 9 Pages |
A comprehensive overview is presented of currently known phase I metabolites of tamoxifen consisting of their systematic name and molecular structure. Reference standards are utilized to elucidate the MSn fragmentation patterns of these metabolites using a linear ion trap mass spectrometer. UV-absorption spectra are recorded and absorption maxima are defined.Serum extracts from ten breast cancer patients receiving 40 mg tamoxifen once daily were qualitatively analyzed for tamoxifen phase I metabolites using a liquid chromatography–tandem mass spectrometry set-up. In total, 19 metabolites have been identified in these serum samples. Additionally a synthetic method for the preparation of the putative metabolite 3′,4′-dihydroxytamoxifen is described.