Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1223255 | Journal of Pharmaceutical and Biomedical Analysis | 2008 | 7 Pages |
Abstract
Solid-phase extraction and column-trapping preconcentration are combined to enhance HPLC–nuclear magnetic resonance (HPLC–NMR) and applied to metabolite profiling in biological samples. Combining the two signal enhancement techniques improved the NMR signal substantially such that we were able to identify 2-hydroxyibuprofen, carboxyibuprofen, and unmetabolized ibuprofen molecules from a small urine sample after a therapeutic dose of ibuprofen. The hyphenated SPE/column-trapping method resulted in an excellent overall signal enhancement of up to 90-fold.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Danijel Djukovic, Emmanuel Appiah-Amponsah, Narasimhamurthy Shanaiah, G.A. Nagana Gowda, Ian Henry, Mike Everly, Brian Tobias, Daniel Raftery,