| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1223399 | Journal of Pharmaceutical and Biomedical Analysis | 2009 | 6 Pages |
Abstract
A reversed-phase high-performance liquid chromatographic (HPLC) method was developed and validated for evaluating the chiral discrimination ability of CYCLOBOND I 2000 DM chiral stationary phase (CSP) towards sertraline and its related enantiomers. The effect of pH, buffer concentration as well as nature of organic modifier, flow rate and temperature on enantioselectivity was investigated. The developed reversed-phase chromatographic conditions were able to separate not only the enantiomers of sertraline but also its process related chiral impurities. The method was validated for determination of enantiomeric purity of sertraline HCl in drug substances and formulations.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
R. Nageswara Rao, M.V.N. Kumar Talluri, Pawan K. Maurya,