Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1223462 | Journal of Pharmaceutical and Biomedical Analysis | 2007 | 5 Pages |
Abstract
Polysaccharides as antigens impose the problem of the proper ELISA assay. The indirect coating using biotin-avidin or lectins are therefore used. We tried to clarify the efficiency of different approaches to this problem. Our experiments clearly demonstrate that direct coating with mannan polysaccharide on high binding ELISA plates is superior to any other combination using any intermediate protein. On the other hand, the direct coating of the normal ELISA plate with mannan was of significantly lower (P = 0.008) efficiency. The use of protein cannot be avoided in normal microplates. All previous statements are supported by experimental data in the paper.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lucia Libjaková, Slavomír Bystrický, Izabela Ližičárová, Ema Paulovičová, Eva Machová,