Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1223463 | Journal of Pharmaceutical and Biomedical Analysis | 2007 | 5 Pages |
Abstract
An isocratic high-performance liquid chromatographic method was developed, optimized and validated for the determination of nystatin in human saliva (UV and fluorescence detection). A reversed-phase Luna⢠C18 column (25 °C), with a mobile phase of MeOH, H2O, and DMF (70:20:10, v/v/v), and a flow-rate of 0.8 ml/min were used. The elution time for nystatin was 5.8 ± 0.2 min. Calibration curves in human saliva were linear from 0.78 to 50 μg/ml. Limits of quantification were 0.78 μg/ml and 0.75 μg/ml for UV and fluorescence detection, respectively. The accuracy and precision values of intra- and inter-day variation studies were within acceptable limits, according to FDA guidelines. The described method has proved to be useful to give accurate measurements of nystatin in real samples.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Juan M. Llabot, Daniel A. Allemandi, Ruben H. Manzo, Marcela R. Longhi,