| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1223868 | Journal of Pharmaceutical and Biomedical Analysis | 2008 | 10 Pages |
Abstract
A reversed-phase high performance liquid chromatographic (RP-HPLC) method for evaluation of purity of tamsulosin in bulk drugs and pharmaceuticals was developed. The separation was accomplished on an Inertsil C18 column using 10 mM ammonium acetate: acetonitrile as a mobile phase in a gradient elution mode. A photodiode array detector set at 280 nm was used for detection. The impurities were identified by ESI-MS–MS. The detection limits were 0.06–0.11 μg/ml. The method was validated with respect to accuracy, precision, linearity, ruggedness and limits of detection and quantification. It finds application not only for monitoring the reactions during the process development but also on quality assurance of tamsulosin.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
R. Nageswara Rao, M.V.N. Kumar Talluri, A. Narasa Raju, Dhananjay D. Shinde, G.S. Ramanjaneyulu,