Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1224148 | Journal of Pharmaceutical and Biomedical Analysis | 2008 | 6 Pages |
Abstract
In this paper, three different HPLC methods for the quantification of thalidomide in tablets were developed and compared. The comparison of a conventional method at 30 °C with two high-temperature methods at 180 °C showed equal results. Using high-temperature HPLC (HT-HPLC), faster analysis times could be achieved. We have also focused on analyte stability and could show that the stationary phase has a pronounced effect on the on-column degradation of thalidomide at high temperatures. Virtually no degradation occurs if a polystyrene divinylbenzene column is used, whereas thalidomide is completely degraded at 180 °C when a carbon clad zirconium dioxide column is used.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sascha Giegold, Maren Holzhauser, Thekla Kiffmeyer, Jochen Tuerk, Thorsten Teutenberg, Maike Rosenhagen, Dorothea Hennies, Torsten Hoppe-Tichy, Bernd Wenclawiak,