Article ID Journal Published Year Pages File Type
1224325 Journal of Pharmaceutical and Biomedical Analysis 2007 5 Pages PDF
Abstract

Saussurea involucrata (Kar. et Kir.) Sch. Bip. (family Asteraceae) is a rare traditional Chinese medicinal herb. A new guaianolide, 11β,13-dihydrodehydrocostuslactone-8-O-[6′-O-acetyl-β-d-glucoside] (1), together with two known guaianolides, namely 11β,13-dihydrodehydrocostuslactone-8-O-β-d-glucoside (2) and 3α-hydroxyl-11β,13-dihydrodehydrocostuslactone-8-O-β-d-glucoside (3), was isolated from the 95% ethanol extract of the whole plants. Their structures were elucidated on the basis of spectral analysis. Their contents in the S. involucrata were determined by HPLC for the first time. Chromatographic separation was achieved on an Agilent Zorbax SB-C18 column (250 mm × 4.6 mm i.d., 5 μm) at 25 °C and the mobile phase was a mixture of acetonitrile and water in a mode of gradient elution detected at 209.8 nm at a flow rate of 1.0 ml/min. This method was validated in terms of selectivity, linearity, precision and accuracy. The linearity ranges were 0.066–0.44 μg for 1, 0.23–2.30 μg for 2, and 0.258–2.58 μg for 3, respectively.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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