| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1224349 | Journal of Pharmaceutical and Biomedical Analysis | 2007 | 6 Pages |
Abstract
A new HPLC-UV method for the determination of the impurity profile of erythromycin is developed. In contrast to the liquid chromatography described in the European Pharmacopoeia the analysis could be performed at a temperature of 25 °C. Erythromycin samples were analysed on an endcapped RP phase with cyanopropyl groups on the surface using gradient elution with 32 mM potassium phosphate buffer pH 8.0 and acetonitrile/methanol (75:25). The aforementioned method shows clear improvements compared to the actual method of the European Pharmacopoeia, which is less selective and sensitive.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
A. Deubel, U. Holzgrabe,